Versadoc 3000 Imaging System with Phosphoimager.
The Macromolecular Equipment Core Facility has a Bio-Rad model 3000 VersaDoc imaging system. This is a quantitative imaging system capable of capturing high resolution digital images from single and multi-color fluorescence, chemiluminescence, chemifluorescence, white light and colorimetric samples.
In conjunction with the VersaDoc, a Personal Molecular Imager has been purchased. This piece of equipment eliminates the need for X-ray film as the visualization of radiolabelled substances can be done quickly and accurately on this phosphoimager. The phosphoimager is capable of imaging and analyzing a broad range of isotopes including 32P, 33P, 35S, 14C, 3H and 125I. The imager uses storage phosphor screen technology that is at least 10 times more sensitive to isotopic emission than x-ray film.
The Quantity One software is capable of a wide range of useful functions. Images can be magnified, annotated, rotated, and resized. All data in the image can be quickly quantitated using the Volume tools provided with the software. As well, the lane based functions can be used to calculate molecular weights, isoelectric points, VNTR's, Rf values, and other values. This software is capable of measuring total and average quantities, determine relative and actual amounts of protein and count colonies in a petri dish.
Application and Analysis:
o Detection and quantitation of nucleic acid-specific stains and labels, including GelStar, ethidium bromide, fluorescein, Radiant Red, SYBR Green, SYBR Gold, and Texas Red; sensitivity is at the picogram level for stained DNA, and at the femtomole level for end-labeled DNA
o Detection and quantitation of protein-specific stains and labels, including Coomassie Brilliant Blue, copper stain, silver stain, SYPRO Orange, SYPRO Red, SYPRO Ruby, and zinc stain
o Detection and quantitation of other stains, including Cy2, Cy3, Green Fluorescent Protein, and rhodamine
o Chemiluminescent, chemifluorescent, colorimetric, and fluorescent blotting applications, including all horseradish peroxidase (HRP)- and alkaline phosphatase (AP)-activated substrates
o Densitometry, including negative, photographic, and X-ray film